Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in biotechnology. Particular areas of interest include the stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceuticals, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology. The journal also presents review articles on subjects in these areas.

分子生物技術發表關于分子生物學在生物技術基礎研究和應用研究中的應用的原始研究論文。特別感興趣的領域包括克隆基因產物的穩定性和表達、細胞轉化,基因克隆系統和生產重組蛋白,蛋白純化和分析,轉基因物種,發育生物學,基因突變分析、DNA指紋圖譜的應用,RNA干擾,和PCR技術,微陣列技術、蛋白質組學、質譜分析、生物信息學、植物分子生物學、微生物遺傳學、基因探針與疾病診斷、藥物、治療劑、疫苗、基因靶向、基因治療、干細胞技術與組織工程、反義技術、蛋白質工程與酶技術、單克隆抗體、糖生物學與糖生物學、農業生物技術。該雜志還發表了關于這些領域主題的評論文章。