BBA Gene Regulatory Mechanisms includes reports that describe novel insights into mechanisms of transcriptional, post-transcriptional and translational gene regulation. Special emphasis is placed on papers that identify epigenetic mechanisms of gene regulation, including chromatin, modification, and remodeling. This section also encompasses mechanistic studies of regulatory proteins and protein complexes; regulatory or mechanistic aspects of RNA processing; regulation of expression by small RNAs; genomic analysis of gene expression patterns; and modeling of gene regulatory pathways. Papers describing gene promoters, enhancers, silencers or other regulatory DNA regions must incorporate significant functions studies.The journal does not favorably review manuscripts identifying a miRNA-target pair without additional insights into the repression mechanism or significant advances in understanding regulatory pathways. In addition, the following elements should be an integral part of the study:?In silico prediction of miRNA targets must be experimentally verified using appropriate luciferase constructs and assays;?To exclude non-functional miRNA/mRNA interactions a reporter system including the whole 3'UTR of the target gene downstream the "luciferase" or GFP should be considered;?Any miRNA modulation should be validated by measuring the expression of the putative protein

BBA基因調控機制包括對轉錄、轉錄后和翻譯基因調控機制的新見解的報告。特別強調的論文，以確定表觀遺傳機制的基因調控，包括染色質，修改和重塑。本節還包括調控蛋白和蛋白復合物的機制研究;RNA處理的調控或機制方面;小rna對表達的調控基因表達模式的基因組分析;以及基因調控通路的建模。描述基因啟動子、增強子、沉默子或其他調節DNA區域的論文必須包含重要的功能研究。如果沒有對抑制機制的深入研究或在理解調控途徑方面的重大進展，該雜志不會對鑒定mirna -靶點對的手稿進行正面評價。此外，下列要素應成為研究的一個組成部分:?在miRNA目標的硅預測中，必須使用合適的熒光素酶構建物和檢測方法進行實驗驗證;?為了排除非功能性miRNA/mRNA相互作用，應該考慮包括“熒光素酶”或GFP下游目標基因的整個3’utr的報告系統;?任何miRNA的調節都應該通過測量假定蛋白的表達來驗證